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  1. Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

  2. Select the sequence database to run searches against. No BLAST database contains all the sequences at NCBI. BLAST databases are organized by informational content (nr, RefSeq, etc.) or by sequencing technique (WGS, EST, etc.). more...

  3. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches.

  4. Local alignments algorithms (such as BLAST) are most often used. A global alignment should only be used on sequences that share significant similarity over most of their extents, and then it will sometimes return a better presentation.

  5. Mar 17, 2014 · BLAST for beginners introduces students to blastn, a commonly used tool for comparing nucleotide sequences (DNA and RNA). This popular tutorial shows how to do a blast search with a nucleotide sequence, highlights information in the search results, and shows how to interpret the E value and alignment scores.

  6. Jul 1, 2004 · Basic Local Alignment Search Tool (BLAST) is a sequence similarity search program that can be used via a web interface or as a stand-alone tool (1,2). There are several types of BLAST to compare all combinations of nucleotide or protein queries with nucleotide or protein databases.

  7. BLAST can rapidly align and compare a query DNA sequence with a database of sequences, which makes it a critical tool in ongoing genomic research.

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