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- Finding a good, quantitative procedure for students to use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to be prone to error and so are unreliable. The floating leaf disk assay technique is both reliable and understandable.
mcb.arizona.edu/outreach/science-home/science-home-photosynthesisScience at Home - Photosynthesis | Molecular and Cellular Biology
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In this fun plant biology project, you will explore how plants use photosynthesis to make energy for themselves with the help of the floating leaf disk assay.
The Floating Leaf Disk Assay for Investigating Photosynthesis. Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable.
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Sep 2, 2019 · Over the years, I have found the floating leaf disk assay technique to be reliable and understandable to students. Once the students are familiar with the technique they can readily design experiments to answer their own questions about photosynthesis.
Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf.
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The point at which 50% of the leaf disks are floating is the point of reference for this procedure. By interpolating from the graph, the 50% floating point is about 11.5 minutes. Using the 50% point provides a greater degree of reliability and repeatability for this procedure.
Over the years, I have found the floating leaf disk assay technique to be reliable and understandable to students. Once the students are familiar with the technique they can readily design experiments to answer their own questions about photosynthesis.
Overview. Plants carry out photosynthesis to produce sugars that they need as an energy source to live and grow. During photosynthesis, oxygen—a gas that many living beings need to survive—is released. This makes photosynthesis one of the most important biological processes on Earth.
