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1. genomicsaotearoa.github.io › hts_workshop_mpi4.3 - Interpreting BLAST results - High Throughput Sequencing

   genomicsaotearoa.github.io › hts_workshop_mpi

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   Interpretting the results of BLAST queries. It is important to remember, like most bioinformatics tools, BLAST has a specific job. In this case sequence alignment to a set of references. BLAST is really good at this job, but it does not offer interpretation of its alignments. Interpretation is completely up to the user on a case by case basis.

2. www.bioteach.ubc.ca › FILES › asmcue2009Lecture/Lab: BLAST - University of British Columbia

   www.bioteach.ubc.ca › FILES › asmcue2009

   sophisticated bioinformatician, it is important that you try not to interpret your BLAST results blindly. Worked Example: Basic Database Similarity Searching Using BLAST There are many different BLAST programs available, but the ones most commonly used for basic database similarity searching are:

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3. Videos

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     NCBI Blast, Sequence Analysis & Result Interpretation: Lecture 2 part 2 by Dr. Muhammad Naveed

     Nov 19, 2019

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     NCBI Blast (how to interpret NCBI Blast results)

     Aug 13, 2020

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     What is BLAST? - Tutorial & Interpretation

     Oct 15, 2020

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4. www.biostars.org › p › 463451Help interpreting BLAST results? (Max score/Percent. Identity ...

   www.biostars.org › p › 463451

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   Sep 25, 2020 · To be more clear, I will report some values so that you will understand better. The query lenght is 370 aa The max score and total score is 340 The query cover is 97% The e-value is 1e-103 And the percentage identity is 47.45%. Now, I understand that is a good allignment, but I would appreciate so much if someone could explain me in a simple ...

5. sequenceserver.com › blog › interpretation-of-blastnInterpreting nucleotide BLAST results - SequenceServer

   sequenceserver.com › blog › interpretation-of-blastn

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   Nucleotide BLAST (blastn) compares one or more nucleotide query sequences to a database of nucleotide sequences. This means comparing DNA to DNA, or DNA to RNA. It is used to compare non-coding sequences, coding sequences, predicted transcripts, messenger RNAs, or genomes. But blastn is not always the most appropriate approach.

6. www.researchgate.net › post › How_can_I_analyze_theHow can I analyze the Blast results properly? - ResearchGate

   www.researchgate.net › post › How_can_I_analyze_the

   Read 8 answers by scientists to the question asked by Houda Amor on Jul 2, 2018. Question. ... Question. Asked 2nd Jul, 2018. Houda Amor. How can I analyze the Blast results properly?

7. environmentalmicrobiome.biomedcentral.comBLAST-QC: automated analysis of BLAST results

   environmentalmicrobiome.biomedcentral.com

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   Aug 12, 2020 · Background The Basic Local Alignment Search Tool (BLAST) from NCBI is the preferred utility for sequence alignment and identification for bioinformatics and genomics research. Among researchers using NCBI’s BLAST software, it is well known that analyzing the results of a large BLAST search can be tedious and time-consuming. Furthermore, with the recent discussions over the effects of ...

8. People also ask

   Can a bioinformatician interpret my BLAST results blindly?

   • As a sophisticated bioinformatician, it is important that you try not to interpret your BLAST results blindly. blastp: compares a protein sequence against a protein sequence database. blastn: compares a nucleotide sequence against a nucleotide sequence database.

   Lecture/Lab: BLAST - University of British Columbia

   www.bioteach.ubc.ca/FILES/asmcue2009/BLAST-background-notes.pdf

   See all results for this question

   Is blast a good bioinformatics tool?

   • It is important to remember, like most bioinformatics tools, BLAST has a specific job. In this case sequence alignment to a set of references. BLAST is really good at this job, but it does not offer interpretation of its alignments. Interpretation is completely up to the user on a case by case basis.

   4.3 - Interpreting BLAST results - High Throughput Sequencing

   genomicsaotearoa.github.io/hts_workshop_mpi/level1/43_blast_interpretation/

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   Does NCBI BLAST affect the correctness of bioinformatics workflows?

   • Shah, Nidhi, et al. “Misunderstood Parameter of NCBI BLAST Impacts the Correctness of Bioinformatics Workflows.” OUP Academic, Oxford University Press, 24 Sept. 2018, academic.oup.com/bioinformatics/article/35/9/1613/5106166.

   BLAST-QC: automated analysis of BLAST results | Environmental

   environmentalmicrobiome.biomedcentral.com/articles/10.1186/s40793-020-00361-y

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   How can bioinformatics improve blast quality control?

   • With the increase in popularity of high-performance computing and new generation sequencing, novel approaches to BLAST quality control and other bioinformatic computational processes are needed to handle the increasing size of datasets, but also to take advantage of the increasing capacity of computing to provide solutions to these problems.

   BLAST-QC: automated analysis of BLAST results | Environmental

   environmentalmicrobiome.biomedcentral.com/articles/10.1186/s40793-020-00361-y

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   How do I choose the right BLAST program?

   • Choosing the right BLAST program is the first issue that must be considered when preparing a BLAST query. The most meaningful alignments are produced when amino acid sequences are compared. This is a result of the complexity of an amino acid sequence in comparison to a nucleotide sequence.

   Lecture/Lab: BLAST - University of British Columbia

   www.bioteach.ubc.ca/FILES/asmcue2009/BLAST-background-notes.pdf

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   How do I interpret BLAST results?

   • Interpreting BLAST results requires you to apply your biological expertise, your understanding of BLAST statistics, and your practical experiences with BLAST. The measure of similarity between two sequences is captured by a scoring scheme in BLAST which is based on scoring matrices.

   Lecture/Lab: BLAST - University of British Columbia

   www.bioteach.ubc.ca/FILES/asmcue2009/BLAST-background-notes.pdf

   See all results for this question

9. erick-samera.gitbook.io › 11.2.2 :: BLAST | Introduction to Applied Bioinformatics - GitBook

   erick-samera.gitbook.io › 1

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   Jun 24, 2023 · NCBI BLAST (Basic Local Alignment Search Tool) is a widely-used bioinformatics software tool that allows for the rapid comparison of nucleotide or protein sequences against a vast array of publicly available sequence databases.