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- As photosynthesis proceeds oxygen is released into the interior of the leaf, which changes the buoyancy--causing the disks to rise. Since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis.
cpb-us-e1.wpmucdn.com/blogs.cornell.edu/dist/3/1009/files/2015/09/Floating-Leaf-Disk-Brad-Williamson.pdfThe Floating Leaf Disk Assay for Investigating Photosynthesis
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How does the floating leaf disk method measure cellular respiration?
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What happens if a leaf disk is placed in bicarbonate solution?
Can photosynthesis occur in a sunken leaf disk?
How to determine the rate of photosynthesis without laboratory equipment?
Why do leaf disks float?
Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf.
You will need to experimentally stop photosynthesis in your leaf disks to measure respiration. Think about the role of oxygen in respiration. How will cellular respiration influence the floating leaf disc assay for photosynthesis? How can you demonstrate that cellular respiration is occurring? What is the role of oxygen in cellular respiration?
Jul 11, 2023 · For photosynthesis to occur in the leaf disks that have had the air sucked out, you will need to provide the leaf disks with light and a good source of CO 2 dissolved in water. For this purpose, you will use a solution of sodium bicarbonate (NaHCO 3 ) in water.
As photosynthesis proceeds oxygen is released into the interior of the leaf, which changes the buoyancy--causing the disks to rise. Since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis. !"#$%&"'()*.
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Disks of leaf tissue are vacuum-infiltrated to replace intercellular air with liquid. As photosynthesis takes place, if the rate of photosynthesis exceeds the rate of cellular respiration, the accumulating oxygen imparts buoyancy to the leaf disk, and it floats.
Jul 11, 2023 · If this leaf disk is placed in a solution with an alternate source of carbon dioxide in the form of bicarbonate ions, then photosynthesis can occur in a sunken leaf disk. As photosynthesis proceeds, oxygen accumulates in the air spaces of the spongy mesophyll and the leaf becomes buoyant and floats.
When the air spaces are infiltrated with a solution the overall density of the leaf disk increases and the disk sinks. The infiltration solution includes a small amount of sodium bicarbonate (NaHCO3) thus enabling the bicarbonate ion to serve as the carbon source for photosynthesis.