Search results
Undesired introduction of impurities
- Contamination is undesired introduction of impurities like chemical, microbial or physical matter, into or onto a starting or intermediating cell culture during sampling, holding, processing, storing, transferring, packaging and transporting.
www.arcjournals.org/pdfs/ijrsb/v6-i4/2.pdfContamination in a Microbiological Laboratory - ARC Journals
People also ask
What is microbial contamination?
What is chemical contamination?
What is contamination in cell culture?
Why is microbial contamination important?
What are biological contaminants?
Do contaminants affect the microbial laboratory?
Study with Quizlet and memorize flashcards containing terms like Define contamination, What is one house keeping task we follow at the beginning and end of each lab to prevent contamination?, What are some ways that the numbers of microorganisms might be decreased in a hospital setting? and more.
Jan 1, 2018 · Objective of our study was to isolate and identify the common laboratory contaminant bacteria with an ultimate goal to reduce false positive culture reports as well as Laboratory acquired...
- Credits Credits & & Acknowledgements Acknowledgements
- Preparation
- Media, sterilisation and disinfection
- Inoculation and other aseptic procedures
- Working with bacteria and yeast
- Essential methods for maintaining, preparing and using cultures
- Part 2: Microbiology in Action Practical activities
- Part
- Safety guidelines
- Relevance Relevance
- Good microbiological laboratory practice (GMLP)
- Hint
- Broken glass
- Splashes on clothing and the skin
- Aerosols
- Pouring a plate
- Storage of media
- Sterilisation vs disinfection
- Choice, preparation and use of disinfectants
- Commonly available disinfectants and their uses
- Essential points
- Using a wire loop
- Flaming procedure
- Hint
- Using a pipette
- Hints
- Flaming the neck of bottles and test tubes
- Hints
- Inoculation using a Pasteur pipette
- Inoculating the Petri dish
- Pouring the plate
- Using a spreader
- Hints
- Working with moulds
- Hint
- Pure cultures
- Maintaining stock cultures
- Checking cultures for contamination
- Cotton wool plugs
- Aseptic transfer of cultures and sterile solutions
- 1. Testing sensitivity to antimicrobial substances
- Using the microscope
- Stained preparations
- Making a smear
- Gram-negative bacteria
- Hints
- General safety considerations
- Microbiology
- Bacteria
- Fungi
- Viruses
- Algae, protozoa (including slime moulds) and lichens
- Suppliers Suppliers of of cultures cultures and and equipment equipment to to schools schools and and colleges colleges
- Specialist Specialist culture culture collections collections
- General
- Steam generation
- Sterilisation
- Unloading
The The SGM Microbiology gratefully Society acknowledges gratefully the acknowledges support from the the support following from sources: the following sources: Kath Crawford (SAPS Scotland) x John Richardson (SSERC) Members of MiSAC x John Schollar (NCBE) John Tranter (CLEAPSS) Editors: Dariel Burdass, John Grainger & Janet Hurst
Safety guidelines Risk assessment Good microbiological laboratory practice (GMLP) Spillage management Aerosols
Preparation of culture media Pouring a plate Storage of media Sterilisation vs disinfection Sterilisation using the autoclave/pressure cooker Sterilisation of equipment and materials Choice, preparation and use of disinfectants
Essential points Using a wire loop Using a pipette Flaming the neck of bottles and test tubes
Streak plate Pour plate Using a spreader Spread plate Working with moulds Incubation
Obtaining suitable cultures Pure cultures Maintaining stock cultures Checking cultures for contamination Preventing contamination of cultures and the environment Aseptic transfer of cultures and sterile solutions Preparing cultures for class use
Testing sensitivity to antimicrobial substances Microscopy Using the microscope Stained preparations Making a smear simple stain differential stain: Gram’s staining method
As well as 1: causing The a familiar Basics range of diseases in animals and plants and problems in food spoilage and deterioration of other materials, microbes are also our ‘invisible allies’. Indeed, life on Earth would not be sustainable without the An benefits introduction that many of them to provide. microbiology, The teaching of such aseptic...
The The small small size size of of microbes microbes and and the the consequent consequent need need to to deal deal with with cultures cultures that that contain contain many many millions millions of of microbial microbial cells cells require require special special procedures procedures for for their their safe safe use. use. Activities Activit...
Reputable specialist supplier or approved environmental sample Degree Degree of of risk risk of of microbial microbial culture; culture; expertise expertise of of teacher; teacher; age age range range of of students Adequate students containment of cultures; class practical work vs. teacher Present demonstration minimum risk; refer to list of suita...
Training in GMLP is aimed at developing proficiency in containing any uncontrolled spread of microbes in order to protect: practical investigations from becoming contaminated with microbes from external sources the operators (students, teachers and tech-nicians) from the very small possibility of infection. (The teacher supervising the practical se...
It is useful to have a spillage kit always at hand ready for use. Suggested components beaker for making fresh disinfectant disposable gloves dustpan paper towelcloth autoclaveroasting bag
Observe an appropriate disposal procedure for broken glass if present. It should be swept carefully into a suitable container, autoclaved and disposed of in a puncture proof container.
Contaminated clothing should be soaked in disinfectant. Splashes on the skin should be treated as soon as possible; washing thoroughly with soap and hot water should be sufficient, but if necessary the skin can be disinfected.
Spillages also carry a risk of generating aerosols (an invisible ‘mist’ of small droplets of moisture) which may contain microbes and might be inhaled. The risk of spillages occurring is lessened by using cultures grown on agar instead of in liquid media whenever possible. Care should also be taken to avoid generating aerosols during practical work...
Collect one bottle of sterile molten agar from the water bath. Hold the bottle in the right hand; remove the cap with the little finger of the left hand. Flame the neck of the bottle. Lift the lid of the Petri dish slightly with the left hand and pour the sterile molten agar into the Petri dish and replace the lid. Flame the neck of the bottle and ...
Store stocks of prepared media at room temperature away from direct sunlight; a cupboard is ideal but an open shelf is satisfactory. Media in vessels closed by cotton wool plugs/plastic caps that are stored for future use will be subject to evaporation at room temperature; avoid wastage by using screw cap bottles. Re-melt stored agar media in a boi...
Sterilisation means the complete destruction of all the micro-organisms including spores, from an object or environment. It is usually achieved by heat or filtration but chemicals or radiation can be used. Disinfection is the destruction, inhibition or removal of microbes that may cause disease or other problems, e.g. spoilage. It is usually achiev...
Specific disinfectants at specified working strengths are used for specific purposes. The choice is now much more straightforward as the range available from suppliers has decreased.
When preparing working strength solutions from stock for class use and dealing with powder form, wear eye protection and gloves to avoid irritant or harmful effects. Disinfectants for use at working strength should be freshly prepared from full strength stock or powder form. Activity of VirKon solution may remain for up to a week (as indicated by r...
There are several essential precautions that must be taken during inoculation procedures to control the opportunities for the contamination of cultures, people or the environment. Operations must not be started until all requirements are within immediate reach and must be completed as quickly as possible. Vessels must be open for the minimum amount...
Wire loops are sterilised using red heat in a Bunsen flame before and after use. They must be heated to red hot to make sure that any contaminating bacterial spores are destroyed. The handle of the wire loop is held close to the top, as you would a pen, at an angle that is almost vertical. This leaves the little finger free to take hold of the cott...
The flaming procedure is designed to heat the end of the loop gradually because after use it will contain culture, which may ‘splutter’ on rapid heating with the possibility of releasing small particles of culture and aerosol formation. Position the handle end of the wire in the light blue cone of the flame. This is the cool area of the flame. Draw...
If a loop does not hold any liquid the loop has not made a complete circle. To correct the problem, first ensure that the loop has been sterilised and then reshape the loop with forceps. Do not use your fingers because of the possibility of puncturing the skin.
Sterile graduated or dropping (Pasteur) pipettes are used to transfer cultures, sterile media and sterile solutions. Remove the pipette from its container/ wrapper by the end that contains a cotton wool plug, taking care to touch no more than the amount necessary to take a firm hold. Fit the teat. Hold the pipette barrel as you would a pen but do n...
A leaking pipette is caused by either a faulty or ill-fitting teat or fibres from the cotton wool plug between the teat and pipette. A dropping Pasteur pipette can be converted to delivering measured volumes by attaching it to a non-sterile syringe barrel by rubber tubing. Commercial dispensing systems are available such as measuring Pasteur pipett...
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
After completion of the holding time, allow the autoclave/pressure cooker to return atmospheric pressure and temperature before starting the opening procedure. Never attempt to reduce either the cooling period with cold water or the time taken for pressure reduction by premature opening of the air cock.
- 2MB
- 46
Contamination. Presence of unwanted microorganisms on inanimate objects or on various body surfaces; in laboratory situations the presence of microbes in specimens. Disease. A disturbance in an individual's state of health preventing the normal functioning of body systems or organs. Infection.
Contamination is undesired introduction of impurities like chemical, microbial or physical matter, into or onto a starting or intermediating cell culture during sampling, holding, processing, storing, transferring, packaging and transporting.
- 464KB
- Endeshaw Abatenh, Birhanu Gizaw, Zerihun Tsegaye
- 7
- 2018
Sep 1, 2020 · In this study, we assessed the bacterial compositions of samples from the surfaces of various sites across different types of biological laboratories. We have qualitatively assessed these possible bacterial contaminants, and found distinct differences in their bacterial community composition.
Oct 25, 2016 · “Decontamination” is a general term that usually refers to a process that makes an item safe to handle, or a space safe to occupy, and can include processes ranging from simple cleaning with soap and water to sterilization.
