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For 4 x 96 preps: High-throughput isolation of DNA from soil samples in less than one day. For 10 preps: Isolate microbial DNA from large quantities of soil; great for samples with low microbial load. For 50 preps: Isolate DNA from tough soil microbes; optimized for use with bead-based homogenizers.
Extracting microbial DNA from soil samples can be challenging. QIAGEN’s new DNeasy PowerSoil Pro Kit is even more effective than our original PowerSoil technology at isolating high yields of pure microbial DNA from all soil types, including compost, clay and top soil.
The QIAGEN RNeasy PowerSoil DNA Elution Kit is designed to co-isolate DNA for the recovery of the total nucleic acid content of the original sample. When used in combination with the RNeasy PowerSoil Total RNA Kit (cat. no. 12866-25), both RNA and DNA can be isolated and eluted into two separate fractions.
The DNeasy PowerSoil Pro Kit comprises a novel and proprietary method for isolating microbial genomic DNA from environmental samples.
Dec 21, 2022 · The eluted DNA is ready for PCR analysis and other downstream applications. The estimated time from start to finish to process two 96 well plates for this protocol is approximately 8...
The new DNeasy PowerSoil Pro Kit was compared with several commercially available sample prep kits. The DNeasy PowerSoil Pro method outperformed all tested kits in compost, clay and top soil samples. Overall DNA yields were up to 8x higher for the DNeasy PowerSoil Pro Kit than for alternative methods (Figure 1).
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The DNA yield was significantly higher from the PowerSoil kit (60±21 ng mg −1), and significantly lower from the Zymo kit (12±16 ng mg −1) compared to all other kits (Fig. 2a). These patterns were largely consistent across all soil types, apart from the dry heath soil, which displayed no significant difference in yield between extraction kits (Fig. S3a).
