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- To measure the rate of photosynthesis, you will replace the air in the spongy mesophyll in your leaf disks with a liquid. This will cause the leaf disks to sink. Then you will put these leaf disks in water with dissolved CO 2 and measure the amount of time it takes for the leaf disks to float.
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The procedure is called the floating leaf disk assay. In this plant biology project, you can use this procedure to investigate which factors affect the rate of photosynthesis. Do you think the light intensity, type of plant, or the temperature matter?
The Floating Leaf Disk Assay for Investigating Photosynthesis. Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable.
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Sep 2, 2019 · For more than 25 years, I have promoted the floating leaf disk assay as one of the best ways for students to explore factors that affect photosynthesis. It’s replicable, it’s cheap, it’s accessible, and most of all it’s fun.
The leaf disk assay measures the rate of oxygen production in the leaf disks, which is a representation for the rate of photosynthesis. When you placed the leaf disks in your syringe, you probably noticed that they all floated on top of the solution.
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Jul 11, 2023 · You will use the "floating leaf disk" method to measure the rate of photosynthesis. To begin, cut several disks from a spinach leaf and put these leaf disks in a cup of water. After you …
The floating disk assay uses the rate at which oxygen is produced or consumed as a measure of the balance between the two reactions. Disks of leaf tissue are vacuum-infiltrated to replace intercellular air with liquid.
The point at which 50% of the leaf disks are floating is the point of reference for this procedure. By interpolating from the graph, the 50% floating point is about 11.5 minutes. Using the 50% point provides a greater degree of reliability and repeatability for this procedure.
