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Jun 4, 2024 · The bacterial growth curve represents the number of live cells in a bacterial population over a period of time. There are four distinct phases of the growth curve: lag, exponential (log), stationary, and death. The initial phase is the lag phase where bacteria are metabolically active but not dividing. The exponential or log phase is a time of ...
- Regina Bailey
This is the aptly named death phase, sometimes called the decline phase. Many cells lyse and release nutrients into the medium, allowing surviving cells to maintain viability and form endospores. A few cells, the so-called persisters, are characterized by a slow metabolic rate. Persister cells are medically important because they are associated ...
Oct 25, 2017 · Bacteria Life Cycle. •••. Updated October 25, 2017. By Joel Michaels. The bacteria life cycle consists of the lag phase, the log or exponential phase, the stationary phase and the death phase. Factors that influence bacterial growth bear heavily on this cycle.
- Joel Michaels
- Stages of the Bacterial Growth Curve. Knowing the difference between the lag, log, stationary, and death phases of a cell growth curve may sound like biology basics but applying that knowledge in active cell culture is a sign of a more fundamental understanding of microbiological processes.
- Lag Phase. This initial cell growth phase is generally characterized by metabolic activity but not growth. The cells utilize media to synthesize the small molecules necessary for replication.
- Log (Exponential Growth) Phase. Once cells enter the exponential growth or log phase, it’s all about the numbers. This is when cells divide by binary fission and the doubling of each generation creates the exponential growth for which the phase is named.
- Stationary Phase. As environmental conditions change and become the limiting factor in growth, cells slow reproduction and enter a stationary phase. In this stage cells are technically still reproducing but it is less than before and roughly equal to the amount of cell death.
Jul 18, 2022 · Figure 8.1.4 8.1. 4: The growth curve of a bacterial culture is represented by the logarithm of the number of live cells plotted as a function of time. The graph can be divided into four phases according to the slope, each of which matches events in the cell. The four phases are lag, log, stationary, and death.
Mar 28, 2024 · During the death phase, the bacterial population declines as cells die off. This decline is primarily caused by two factors: the accumulation of toxic waste products and autolytic enzymes and the exhaustion of available nutrients. In the death phase, the number of viable bacteria decreases continuously and exponentially.
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Mar 12, 2021 · The enlarged view shows the square within which bacteria (red cells) are counted. If the coverslip is 0.2 mm above the grid and the square has an area of 0.04 mm 2, then the volume is 0.008 mm 3, or 0.000008 mL. Since there are 10 cells inside the square, the density of bacteria is 10 cells/0.000008 mL, which equates to 1,250,000 cells/mL.
