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Jun 4, 2024 · The bacterial growth curve represents the number of live cells in a bacterial population over a period of time. There are four distinct phases of the growth curve: lag, exponential (log), stationary, and death. The initial phase is the lag phase where bacteria are metabolically active but not dividing. The exponential or log phase is a time of ...
- Regina Bailey
- Stages of the Bacterial Growth Curve. Knowing the difference between the lag, log, stationary, and death phases of a cell growth curve may sound like biology basics but applying that knowledge in active cell culture is a sign of a more fundamental understanding of microbiological processes.
- Lag Phase. This initial cell growth phase is generally characterized by metabolic activity but not growth. The cells utilize media to synthesize the small molecules necessary for replication.
- Log (Exponential Growth) Phase. Once cells enter the exponential growth or log phase, it’s all about the numbers. This is when cells divide by binary fission and the doubling of each generation creates the exponential growth for which the phase is named.
- Stationary Phase. As environmental conditions change and become the limiting factor in growth, cells slow reproduction and enter a stationary phase. In this stage cells are technically still reproducing but it is less than before and roughly equal to the amount of cell death.
Mar 28, 2024 · During the death phase, the bacterial population declines as cells die off. This decline is primarily caused by two factors: the accumulation of toxic waste products and autolytic enzymes and the exhaustion of available nutrients. In the death phase, the number of viable bacteria decreases continuously and exponentially.
Feb 1, 2024 · Bacterial cells in the stationary phase exhibit enhanced stress resistance mechanisms, allowing them to survive adverse conditions. The stationary phase is a critical period in microbial growth, as it sets the stage for the subsequent decline in population size. Stage 4: Death Phase. The death phase marks the final stage of the microbial growth ...
The Death Phase. As a culture medium accumulates toxic waste and nutrients are exhausted, cells die in greater and greater numbers. Soon, the number of dying cells exceeds the number of dividing cells, leading to an exponential decrease in the number of cells (Figure 7.5). This is the aptly named death phase, sometimes called the decline phase ...
Jul 18, 2022 · Figure 8.1.4 8.1. 4: The growth curve of a bacterial culture is represented by the logarithm of the number of live cells plotted as a function of time. The graph can be divided into four phases according to the slope, each of which matches events in the cell. The four phases are lag, log, stationary, and death.
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During the death phase, the number of viable cells decreases geometrically (exponentially), essentially the reverse of growth during the log phase. This phase continues until the population is diminished to a tiny fraction of the number of cells in the previous phase or until the population dies out entirely. Image 1: Bacterial growth curve ...
