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- The baking soda provides the carbon dioxide that the leaf needs for photosynthesis. The leaf disks are then sunk in the baking soda solution and exposed to light. As the plant leaf photosynthesizes, oxygen is produced that accumulates as oxygen gas bubbles on the outside of the leaf disk.
www.sciencebuddies.org/science-fair-projects/project-ideas/PlantBio_p053/plant-biology/photosynthesis-leaf-disk-assay
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How does the rate of photosynthesis affect the floatation of a disk?
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How do plants convert light energy into chemical energy? What happens during the floating leaf disk assay? What environmental factors do you think affect the rate of photosynthesis? How is photosynthesis connected to cellular respiration?
In this lab, leaf disks will be used to assay the rate of photosynthesis under various light conditions. The leaf disks will be put into a solution of baking soda (sodium bicarbonate, NaHCO3) and detergent.
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When the air spaces are infiltrated with a solution the overall density of the leaf disk increases and the disk sinks. The infiltration solution includes a small amount of sodium bicarbonate (NaHCO3) thus enabling the bicarbonate ion to serve as the carbon source for photosynthesis.
There are many factors that affect the rate of photosynthesis or how fast plants make oxygen. For example, you can change the temperature of the baking soda solution. Do your leaf disks start to float sooner in a colder or a warmer baking soda solution? Test how the intensity of the light affects photosynthesis.
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influence the floating leaf disc assay for photosynthesis? How can you demonstrate that cellular respiration is occurring? What is the role of oxygen in cellular respiration? You will plot the percent leaf disks floating versus time to be able to calculate the rates of respiration and photosynthesis.
In the first part, students use the leaf disk assay to explore how plants make energy using photosynthesis. In the second part, students design and conduct experiments that use the leaf disk assay to investigate several variables that have the potential to affect the rate of photosynthesis.
Students punch out small discs from leaves, and float them in a syringe of sodium hydrogen carbonate solution. Once gas is evolved by photosynthesis, the leaf discs rise and fall. The resource is suitable for use at Key Stage Three, GCSE and A level.
