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How do plants convert light energy into chemical energy? What happens during the floating leaf disk assay? What environmental factors do you think affect the rate of photosynthesis? How is photosynthesis connected to cellular respiration?
At any given point in this experiment, the number of floating leaf disks is an indirect measurement of the net rate of photosynthesis. In photosynthesis, plants use energy from the sun, water, and carbon dioxide (CO 2) from the air to store carbon and energy in the form of glucose molecules.
As photosynthesis proceeds, oxygen is released into the interior of the leaf which changes its buoyancy causing the disks to rise. Since cellular respiration is taking place at the same time within the leaf, consuming the oxygen generated by photosynthesis, the rate that the disks rise is an indirect measurement of the net rate
Part A: Aim – to explore a method that uses leaf discs to measure rate of photosynthesis. Part B: Aim – to measure the effect of light colour (wavelength) on the rate of photosynthesis. Aim – to measure the effect of carbon dioxide concentration on the rate of pho. These practical activities can be used to support assessment of Unit 3 outcome 2.
Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.
- Anne Marie Helmenstine, Ph.D.
You will need to experimentally stop photosynthesis in your leaf disks to measure respiration. Think about the role of oxygen in respiration. How will cellular respiration influence the floating leaf disc assay for photosynthesis? How can you demonstrate that cellular respiration is occurring? What is the role of oxygen in cellular respiration?
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Can leaf discs be used to investigate photosynthesis?
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Why do spinach leaf disks rise and fall in a baking soda solution?
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Why do leaf disks float?
Jul 27, 2016 · This lab, intended for AP Biology students investigates photosynthesis. Students use a straw to cut small discs from spinach. Then, they use a syringe to remove the air from the air spaces in the leaves. When leaf discs are places in a sodium bicarbonate solution, they will sink to the bottom.
