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- As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float.
knowledge.carolina.com/discipline/earth-environmental/photosynthesis-and-floating-leaf-disks/Photosynthesis and Floating Leaf Disks - Carolina Knowledge ...
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What is a floating leaf disk assay?
In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. In the next step, a vacuum is used to replace the air pockets within the leaf structure with a baking soda (bicarbonate) solution.
When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis. Accumulation of O 2 on the disks causes them to float.
Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.
- Anne Marie Helmenstine, Ph.D.
Jul 11, 2023 · To measure the rate of photosynthesis, you will replace the air in the spongy mesophyll in your leaf disks with a liquid. This will cause the leaf disks to sink. Then you will put these leaf disks in water with dissolved CO 2 and measure the amount of time it takes for the leaf disks to float.
Observe the time it takes for leaf disks to rise to the surface. As photosynthesis occurs, oxygen accumulates in the disks, causing them to float. Record the time it takes for each disk to float to the top.
To measure the rate of photosynthesis, you will first suck the air out of the spongy mesophyll in your leaf disks, so the leaf disks will not float. Then you will put these leaf disks in water with dissolved CO2 and measure the amount of time it takes for the leaf disks to float.
The purpose of the vacuum is to force out all air in the inner spaces of the leaf disks. The solution will now have room to flood in and saturate the leaf disk.
