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- As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float.
knowledge.carolina.com/discipline/earth-environmental/photosynthesis-and-floating-leaf-disks/Photosynthesis and Floating Leaf Disks - Carolina Knowledge ...
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Why do leaf disks float?
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How do floating leaf disks measure the net rate of photosynthesis?
What happens if you put a vacuum on a leaf disk?
In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. In the next step, a vacuum is used to replace the air pockets within the leaf structure with a baking soda (bicarbonate) solution.
This is because leaves have air in the spaces between cells, which helps them collect CO 2 gas from their environment to use in photosynthesis. When you apply a gentle vacuum to the leaf disks in solution, this air is forced out and replaced with solution, causing the leaves to sink.
Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.
- Anne Marie Helmenstine, Ph.D.
Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
Jul 11, 2023 · To measure the rate of photosynthesis, you will replace the air in the spongy mesophyll in your leaf disks with a liquid. This will cause the leaf disks to sink. Then you will put these leaf disks in water with dissolved CO 2 and measure the amount of time it takes for the leaf disks to float.
To measure the rate of photosynthesis, you will first suck the air out of the spongy mesophyll in your leaf disks, so the leaf disks will not float. Then you will put these leaf disks in water with dissolved CO2 and measure the amount of time it takes for the leaf disks to float.
Sep 2, 2019 · What happens if you place the floating leaf disks back in the dark? Without light, we can assume that photosynthesis is not going to take place but will the leaf disks still do respiration? Will they use the oxygen in the leaf spaces and if they do will the leaves sink?
