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In this fun plant biology project, you will explore how plants use photosynthesis to make energy for themselves with the help of the floating leaf disk assay.
When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis. Accumulation of O 2 on the disks causes them to float.
Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.
- Anne Marie Helmenstine, Ph.D.
Do your leaf disks start to float sooner in a colder or a warmer baking soda solution? Test how the intensity of the light affects photosynthesis. Place the cup closer to your light source and farther away.
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Jul 11, 2023 · You will use the "floating leaf disk" method to measure the rate of photosynthesis. To begin, cut several disks from a spinach leaf and put these leaf disks in a cup of water. 1. Do your leaf disks float? Use the information in this diagram of a cross-section of a leaf to explain why a leaf disk would float. 2. Where does photosynthesis occur ...
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What is a floating leaf disk assay?
Jul 12, 2023 · Leaf disks normally float. What caused the leaf disks to sink? To measure the rate of photosynthesis, you replaced the air in the spongy mesophyll in your leaf disks with a liquid.
