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This video is about photosynthesis and covers the AP Biology lab "What factors affect the rate of photosynthesis in living leaves".
- 9 min
- 23.3K
- Madden Science
Feb 21, 2024 · In this method video, Molly takes us into the lab to perform a leaf disk assay to measure the rate of photosynthesis in spinach leaves. View this video (and more like it) on LabXchange: https...
- 8 min
- 11.4K
- LabXchange
Aug 6, 2020 · Photosynthesis is observed in this activity using a sodium bicarbonate solution and leaf discs from a non-hairy, non-waxy plant.
- 4 min
- 634
- QEST Inc
- Conducting The Floating Leaf Disk Assay
- Analyzing Your Results
- Changing Variables to Investigate Photosynthesis
Before you start testing different variables, conduct the leaf disk assay once to get familiar with the procedure. The video Measure Photosynthesis with Floating Leavesdemonstrates how to do each individual step. You will do all your experiments in triplicates after your test run; the following instructions are for the three trials of your experime...
For each cup, create a graph from your data table that shows the number of floating leaf disks over time. Plot the time on the x-axis and the number of floating leaf disks on the y-axis. An example...
Now that you are familiar with the leaf disk assay procedure and data analysis, you are ready to start your own investigations.Choose one variable that you want to investigate. You can find some suggestions in Table 2. The table also provides some possible variations for each variable that you can test.The leaf disks will be floating. Make sure they are all in the water. Close the tip and pull back on the syringe plunger to create a vacuum. Hold for 10 seconds. This process helps remove air from the spongy mesophyll layer of the leaf disks, causing them to sink in the solution. Continue until all of the disks have sunk in the syringe.
Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
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Sep 2, 2019 · Leaf disks float, normally. When the air spaces are infiltrated with the solution the overall density of the leaf disk increases and the disk sinks. The infiltration solution includes a small amount of Sodium bicarbonate. Bicarbonate ion serves as the carbon source for photosynthesis.
