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Why do leaf disks float up?
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In this fun plant biology project, you will explore how plants use photosynthesis to make energy for themselves with the help of the floating leaf disk assay.
When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis. Accumulation of O 2 on the disks causes them to float.
Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
Jun 30, 2019 · The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. Oxygen released from the leaves forms tiny bubbles that cause the leaves to float.
- Anne Marie Helmenstine, Ph.D.
The leaf disk assay measures the rate of oxygen production in the leaf disks, which is a representation for the rate of photosynthesis. When you placed the leaf disks in your syringe, you probably noticed that they all floated on top of the solution.
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During cellular respiration, plants use the sugar they produced during photosynthesis as an energy source, together with the oxygen, to create carbon dioxide and water. This means the oxygen bubbles that make the leaf disks float get consumed over time and the leaf disks sink.
The leaf disks sink due to the baking soda and soap. The baking soda creates carbon dioxide, and fills the air spaces of the leaves, making it more dense. The soap breaks down the cuticles, preventing H2O from entering.
