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- The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. Oxygen released from the leaves forms tiny bubbles that cause the leaves to float.
www.thoughtco.com/floating-spinach-disks-photosynthesis-demonstration-604256Floating Spinach Disks Photosynthesis Demonstration - ThoughtCo
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This video is about photosynthesis and covers the AP Biology lab "What factors affect the rate of photosynthesis in living leaves".
- 9 min
- 23.3K
- Madden Science
Plant material will generally float in water. This is because leaves have air in the spaces between cells, which helps them collect CO 2 gas from their environment to use in photosynthesis. When you apply a gentle vacuum to the leaf disks in solution, this air is forced out and replaced with solution, causing the leaves to sink.
- Conducting The Floating Leaf Disk Assay
- Analyzing Your Results
- Changing Variables to Investigate Photosynthesis
Before you start testing different variables, conduct the leaf disk assay once to get familiar with the procedure. The video Measure Photosynthesis with Floating Leavesdemonstrates how to do each individual step. You will do all your experiments in triplicates after your test run; the following instructions are for the three trials of your experime...
For each cup, create a graph from your data table that shows the number of floating leaf disks over time. Plot the time on the x-axis and the number of floating leaf disks on the y-axis. An example...
Now that you are familiar with the leaf disk assay procedure and data analysis, you are ready to start your own investigations.Choose one variable that you want to investigate. You can find some suggestions in Table 2. The table also provides some possible variations for each variable that you can test.Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.
- Anne Marie Helmenstine, Ph.D.
Do your leaf disks start to float sooner in a colder or a warmer baking soda solution? Test how the intensity of the light affects photosynthesis. Place the cup closer to your light source and farther away.
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Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
The leaf disks will be floating. Make sure they are all in the water. Close the tip and pull back on the syringe plunger to create a vacuum. Hold for 10 seconds. This process helps remove air from the spongy mesophyll layer of the leaf disks, causing them to sink in the solution. Continue until all of the disks have sunk in the syringe.
