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      • Turn on the light and record the time. As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float.
      knowledge.carolina.com/discipline/earth-environmental/photosynthesis-and-floating-leaf-disks/
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  2. When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis. Accumulation of O 2 on the disks causes them to float.

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    Do you know how plants get the energy they need to grow and reproduce? They do not eat like humans and animals do; they make their own food with a process called photosynthesis.During photosynthesis, plants convert light, water, and carbon dioxide from the air into oxygen and sugars. In this activity, you will be able to observe the oxygen producti...

    The leaf disk assay measures the rate of oxygen production in the leaf disks, which is a representation for the rate of photosynthesis. When you placed the leaf disks in your syringe, you probably noticed that they all floated on top of the solution. This is because leaves have air pockets within their structure to facilitate gas exchange between d...

    Every living organism needs energy to grow and reproduce. Humans and animals eat foods with carbohydrates, proteins, and fats to produce the energy they need to survive. But plants do not eat. They make their own energy source in the form of energy-rich carbohydrates (sugars) through a process called photosynthesis. Photosynthesis is a multistep, e...

    There are many factors that affect the rate of photosynthesis or how fast plants make oxygen. For example, you can change the temperature of the baking soda solution. Do your leaf disks start to fl...
    Test how the intensity of the light affects photosynthesis. Place the cup closer to your light source and farther away. How do your results change?
    Different plants have different amounts of chlorophyll, which are the pigments that absorb the sunlight during photosynthesis. How do different plants compare in your leaf disk assay?
    Plants need carbon dioxide for photosynthesis that you provide in the baking soda solution. Find out if changing the baking soda concentration has an effect on photosynthesis!

    Links

    1. Scitable by Nature Education; Photosynthetic cells 2. BBC: Factors affecting photosynthesis

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  3. In a process called photosynthesis, plants convert light energy, water, and carbon dioxide into oxygen and sugar. They can then use the sugar as an energy source to fuel their growth. Scientists have found an easy way to measure the rate of photosynthesis in plants. The procedure is called the floating leaf disk assay.

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  4. Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.

    • Anne Marie Helmenstine, Ph.D.
  5. Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.

  6. Jul 11, 2023 · You will use the "floating leaf disk" method to measure the rate of photosynthesis. To begin, cut several disks from a spinach leaf and put these leaf disks in a cup of water. 1. Do your leaf disks float? Use the information in this diagram of a cross-section of a leaf to explain why a leaf disk would float. 2. Where does photosynthesis occur ...

  7. Observe the time it takes for leaf disks to rise to the surface. As photosynthesis occurs, oxygen accumulates in the disks, causing them to float. Record the time it takes for each disk to float to the top.

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