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- As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float.
knowledge.carolina.com/discipline/earth-environmental/photosynthesis-and-floating-leaf-disks/Photosynthesis and Floating Leaf Disks - Carolina Knowledge ...
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In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. In the next step, a vacuum is used to replace the air pockets within the leaf structure with a baking soda (bicarbonate) solution.
This is because leaves have air in the spaces between cells, which helps them collect CO 2 gas from their environment to use in photosynthesis. When you apply a gentle vacuum to the leaf disks in solution, this air is forced out and replaced with solution, causing the leaves to sink.
Jul 11, 2023 · Which product of photosynthesis will accumulate in the spongy mesophyll and cause the leaf disks to float? For photosynthesis to occur in the leaf disks that have had the air sucked out, you will need to provide the leaf disks with light and a good source of CO 2 dissolved in water.
Do your leaf disks start to float sooner in a colder or a warmer baking soda solution? Test how the intensity of the light affects photosynthesis. Place the cup closer to your light source and farther away.
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Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
Biology. Floating Leaf Disk Photosynthesis Lab. By Kim. I use this experiment to reinforce what we’ve been learning in the photosynthesis lessons. I will be using principles of experimental design, scientific method, and CER as well. The floating leaf disk photosynthesis lab is pretty accessible.
Why do you think the leaf disks started to float over time? Students most likely observed that the leaf disks in the "with baking soda, light" cup all started to float after a while. If they took a closer look at the leaf disk surface, they might have noticed gas bubbles accumulating over time.
