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- As photosynthesis proceeds, the oxygen gas produced will displace the liquid from the intracellular space, and the specific gravity will decrease, and the disks will float. The time required for the disks to float is inversely related to the rate of photosynthesis. The source of the carbon dioxide in this process is the bicarbonate solution.
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When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis. Accumulation of O 2 on the disks causes them to float.
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- Condiment Diver
- Conducting The Floating Leaf Disk Assay
- Analyzing Your Results
- Changing Variables to Investigate Photosynthesis
Before you start testing different variables, conduct the leaf disk assay once to get familiar with the procedure. The video Measure Photosynthesis with Floating Leavesdemonstrates how to do each individual step. You will do all your experiments in triplicates after your test run; the following instructions are for the three trials of your experime...
For each cup, create a graph from your data table that shows the number of floating leaf disks over time. Plot the time on the x-axis and the number of floating leaf disks on the y-axis. An example...
Now that you are familiar with the leaf disk assay procedure and data analysis, you are ready to start your own investigations.Choose one variable that you want to investigate. You can find some suggestions in Table 2. The table also provides some possible variations for each variable that you can test.Jul 11, 2023 · This will cause the leaf disks to sink. Then you will put these leaf disks in water with dissolved CO 2 and measure the amount of time it takes for the leaf disks to float. Which product of photosynthesis will accumulate in the spongy mesophyll and cause the leaf disks to float?
Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.
- Anne Marie Helmenstine, Ph.D.
For photosynthesis to occur in the leaf disks that have had the air sucked out, you will need to provide the leaf disks with light and a good source of CO 2 dissolved in water. For this purpose, you will use a solution of sodium bicarbonate (NaHCO 3) in water. Sodium bicarbonate reacts with water as follows:
Timer. Stirring rod or spoon. Hypothesis: Students can hypothesize whether the presence of baking soda and light will increase, decrease, or have no effect on the rate of photosynthesis in spinach leaf disks. Review the inputs and outputs of photosynthesis before asking students to make a hypothesis.
Why do you think the leaf disks started to float over time? Students most likely observed that the leaf disks in the "with baking soda, light" cup all started to float after a while. If they took a closer look at the leaf disk surface, they might have noticed gas bubbles accumulating over time.
