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This video is about photosynthesis and covers the AP Biology lab "What factors affect the rate of photosynthesis in living leaves".
- 9 min
- 23.3K
- Madden Science
Aug 6, 2020 · Photosynthesis is observed in this activity using a sodium bicarbonate solution and leaf discs from a non-hairy, non-waxy plant.
- 4 min
- 634
- QEST Inc
Feb 21, 2024 · In this method video, Molly takes us into the lab to perform a leaf disk assay to measure the rate of photosynthesis in spinach leaves. View this video (and more like it) on LabXchange: https...
- 8 min
- 11.4K
- LabXchange
- Conducting The Floating Leaf Disk Assay
- Analyzing Your Results
- Changing Variables to Investigate Photosynthesis
Before you start testing different variables, conduct the leaf disk assay once to get familiar with the procedure. The video Measure Photosynthesis with Floating Leavesdemonstrates how to do each individual step. You will do all your experiments in triplicates after your test run; the following instructions are for the three trials of your experime...
For each cup, create a graph from your data table that shows the number of floating leaf disks over time. Plot the time on the x-axis and the number of floating leaf disks on the y-axis. An example...
Now that you are familiar with the leaf disk assay procedure and data analysis, you are ready to start your own investigations.Choose one variable that you want to investigate. You can find some suggestions in Table 2. The table also provides some possible variations for each variable that you can test.Do your leaf disks start to float sooner in a colder or a warmer baking soda solution? Test how the intensity of the light affects photosynthesis. Place the cup closer to your light source and farther away.
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Observe the time it takes for leaf disks to rise to the surface. As photosynthesis occurs, oxygen accumulates in the disks, causing them to float. Record the time it takes for each disk to float to the top.
When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis. Accumulation of O 2 on the disks causes them to float.
