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  1. The present update deals with vigorous developments in mesoscopy, microscopy and nanoscopy methods that have been translated to imaging of plant subcellular compartments, cells, tissues and organs over the past 3 years with the aim to report recent applications and reasonable expectations from current light-sheet fluorescence microscopy (LSFM) and super-resolution microscopy (SRM) modalities.

    • Cell Structure
    • Environmental Impacts
    • Disease
    • Taxonomy
    • Field Work and Education

    Placing a very thin sliver of plant material under a microscope offers the observer the opportunity to see cell structures within the plant. With increased magnification, curious explorers can see that cells are more than just blobs with a border and a center, but complex structures that carry out the following important functions necessary to a pl...

    Looking at plant cells under a microscope can reveal evidence of environmental stresses and damage. While a plant may look healthy at the macroscopic level, at the cellular level it may be suffering damage from environmental stresses such as climate change or toxic pollution. Microscopic observation can be an early warning system of crop stresses, ...

    Like environmental impacts, botanists can observe and identify plant diseases using microscopes. Parasites, insects, bacteria, fungi, and viruses may cause disease and death in plants up to and including old-growth trees. Identifying the source of a plant’s distress could help with early intervention to treat the underlying cause, or to protect nei...

    During his lifetime, Linnaeus never traveled beyond northern Europe. But as European nations developed economic systems based on seafaring and global trade, students and admirers of Linnaeus went along for the ride and returned samples of previously unknown plant species to him for classification. Linnaeus’s work of classifying and organizing the n...

    Until recently, samples had to be delivered to labs that harbored the microscopes used to examine them. Now, however, portable microscopes make field study of plant specimens possible. Foldable microscopescan tuck into a backpack or pocket. Researchers can prepare slides at the site where a plant is growing and observe a specimen’s live attributes ...

    • James Cybulski
  2. Jun 18, 2021 · Microscopy examination of Saxifraga plants also turned up some novel cell structures. “ Saxifraga scardica has a special tissue surrounding the leaf edge that appears to deflect light from the edge into the leaf,” says Wightman. “The cells appear to be producing novel cell wall structures to achieve this deflection.

  3. Another solution is to use highly sensitive microscopy techniques to limit the amount of light treatment and thus the activation of light-sensitive pathways (i.e. spinning disk confocal microscopy, light sheet fluorescence microscopy—see Table 1). Finally, for very long imaging experiments, it may be necessary to add lighting above the microscope stage to mimic the day/night cycle.

  4. Schlau-Cohen fires lasers at the proteins and uses special microscopes to understand how they interact with light—how they absorb it, what happens to the light as it moves around inside the proteins, and how some of it gets converted into heat. For instance, Schlau-Cohen discovered that one of the proteins she’s interested in has two ways ...

  5. Feb 20, 2019 · This special issue of Microscopy aims to provide an overview of how modern imaging technologies have advanced the field of plant sciences. This issue contains seven review articles and one technical report, covering from imaging plant organs to molecular machineries using both light and electron microscope methods and micro-CT approaches.

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  7. Mar 28, 2016 · Despite its clear advantages over confocal and wide field microscopy, LSFM faces a number of specific challenges. Penetration into scattering tissues and the thickness and uniformity of the light sheet are the main determinants of image quality. As such, an important limitation of light sheet microscopy is light scattering.

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