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GUS plc was a FTSE 100 retailing, manufacturing and financial conglomerate based in the United Kingdom. GUS was an abbreviation of Great Universal Stores, the company's name before 2001, while it was also known as the Glorious Gussies amongst stockbrokers.
- 1 Kinetic Fluorometric Assay
- 2 Endpoint Fluorometric Assay
- 3 Protein Assay
1. Aliquot approx 2–5 mg (50–200 seeds) of Arabidropsis seeds into a 1.5-mL Eppendorf tube and grind thoroughly in a 150 µL GUS extraction buffer using a motorized micropestle. Remember to assay no...2. Centrifuge the homogenate in an Eppendorf centrifuge, at maximum speed, for 5 min, then place on ice.3. Switch on the Fluoroskan II, as it requires at least 30 min to warm up.4. Aliquot 100 µL of substrate solution into each well. Prepare sufficient wells to assay each sample in duplicate (see Note 7).1. In an Eppendorf tube, mix 100 µL of sample extract with 400 µL of 1 mM4-MUG made up in GUS extraction buffer.2. Place the Eppendorf tube at 37°C. After approx 2 min, remove a 100-µL aliquot of the reaction mix and add this to an equal volume of 0.2MNa2CO3 (stopping reagent). This timepoint is T= 0. Repeat...3. Measure the fluorescence of the stopped reactions, plot against time, and calculate the rate. Note: The highly alkaline stopping reagent results in significant amplification of the fluorescence...1. First prepare a calibration curve using bovine serum albumin (BSA) standards. In 1.6-mL disposable cuvets aliquot 1, 2, 4, 6, 8, 10, and 12 µL of BSA from an aqueous solution of 1 mg/mL. Add 5 µ...2. At the same time, place 5 µL of extract in a 1.6-mL disposable cuvet. Make the volume up to 800 µL with distilled water and add 200 µL of Bradford reagent. Mix by inversion, and leave at room te...3. Standardize samples by expressing GUS activities as pmol 4-MU/pg protein/min (see Note 9).- Gillian A. Hull, Martine Devic
- 1995
One of the earliest reporter genes used in plants is gusA (or uidA), which encodes β-glucuronidase (GUS) (β-D-glucuronoside glucuronosohydrolase, E.C. 3.2.1.31).
- Kye-Won Kim, Vincent R. Franceschi, Laurence B. Davin, Norman G. Lewis
- 2006
In the unfinished lab, Gus twisted the electrical cord to make it easy to disconnect, then carefully counted his steps to the excavator where he hid the gun. I think he knows Lalo will figure out the lab and is preparing for a fight in the dark.
Gustavo Fring is a fictional character portrayed by Giancarlo Esposito in the Breaking Bad franchise, serving as one of the main antagonists in the crime drama series Breaking Bad and a major character in its prequel Better Call Saul.
Aug 10, 2016 · Guide to the Alliance mission alert “Shining in the Darkness” released in SWTOR 4.7 allowing you to recruit the classic companions Guss Tuno! It’s a short mission, but there is that typical Guss-humour all over it.
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Aug 5, 2023 · The β-glucuronidase (GUS) reporter gene system is an important technique with versatile uses in the study of flower development in a broad range of species. Transcriptional and translational GUS fusions are used to characterize gene and protein expression...
