Search results
Both reliable and understandable
slideplayer.com
- Finding a good, quantitative procedure for students to use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to be prone to error and so are unreliable. The floating leaf disk assay technique is both reliable and understandable.
mcb.arizona.edu/outreach/science-home/science-home-photosynthesisScience at Home - Photosynthesis | Molecular and Cellular Biology
People also ask
How reliable is the floating leaf disk assay technique?
Can a floating leaf disk test be used for photosynthesis?
What is a leaf disk assay?
What is a floating disk assay for photosynthesis?
How long does it take a leaf disk to float?
Do leaf disks float or sink?
Sep 2, 2019 · Over the years, I have found the floating leaf disk assay technique to be reliable and understandable to students. Once the students are familiar with the technique they can readily design experiments to answer their own questions about photosynthesis.
Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf.
The Floating Leaf Disk Assay for Investigating Photosynthesis. Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable.
- 1MB
- 8
Over the years, I’ve found that the floating leaf disk assay technique to be reliable and understandable to students. Once the students are familiar with the technique they can readily design experiments to answer their own questions about photosynthesis.
The leaf disk assay measures the rate of oxygen production in the leaf disks, which is a representation for the rate of photosynthesis. When you placed the leaf disks in your syringe, you probably noticed that they all floated on top of the solution.
- (96)
Over the years, I have found the floating leaf disk assay technique to be reliable and understandable to students. Once the students are familiar with the technique they can readily design experiments to answer their own questions about photosynthesis.
The point at which 50% of the leaf disks are floating (the median) is the point of reference for this procedure. By extrapolating from the graph, the 50% floating point is about 11.5 minutes. Using the 50% point provides a greater degree of reliability and repeatability for this procedure.
