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- Scientists have found an easy way to measure the rate of photosynthesis in plants. The procedure is called the floating leaf disk assay. In this plant biology project, you can use this procedure to investigate which factors affect the rate of photosynthesis.
www.sciencebuddies.org/science-fair-projects/project-ideas/PlantBio_p053/plant-biology/photosynthesis-leaf-disk-assay
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How do floating leaf disks measure the net rate of photosynthesis?
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How to determine the rate of photosynthesis without laboratory equipment?
What is a leaf disk assay?
Why do leaf discs rise and fall?
How do you use leaf disks in a lab?
Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf.
In this fun plant biology activity, you will use a floating leaf disk experiment to measure how quickly plants can make oxygen from photosynthesis.
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At any given point in this experiment, the number of floating leaf disks is an indirect measurement of the net rate of photosynthesis. In photosynthesis, plants use energy from the sun, water, and carbon dioxide (CO 2) from the air to store carbon and energy in the form of glucose molecules.
This SAPS teaching resource provides a fun way for students to get hands-on when investigating photosynthesis, using an easily quantifiable and reliable procedure. Students punch out small discs from leaves, and float them in a syringe of sodium hydrogen carbonate solution.
Possible investigations could include the rate of photosynthesis in sun and shade plants, and at different light intensities. Students punch out small discs from leaves, and float them in a syringe of sodium hydrogen carbonate solution.
Jul 11, 2023 · In this section, students learn how to use the floating leaf disk method to measure the rate of net photosynthesis (i.e. the rate of photosynthesis minus the rate of cellular respiration). They compare the rate of net photosynthesis in water vs. a solution of sodium bicarbonate.
In the first part, students use the leaf disk assay to explore how plants make energy using photosynthesis. In the second part, students design and conduct experiments that use the leaf disk assay to investigate several variables that have the potential to affect the rate of photosynthesis.
