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Sep 30, 2022 · Insulin sensitivity was assessed by hyperinsulinaemic–euglycaemic clamps with d-[6,6-2 H 2]glucose, hepatic and skeletal muscle energy metabolism were assessed by 1 H/ 31 P-magnetic resonance spectroscopy, while high-resolution respirometry measured skeletal muscle and white adipose tissue (WAT) mitochondrial capacity. All participants and people assessing the outcomes were blinded.
- 10.1007/s00125-022-05797-0
- 2023
- Diabetologia. 2023; 66(1): 57-69.
- Volunteers
- Randomisation
- Experimental Protocol
- Indirect Calorimetry
- Skeletal Muscle and Wat Biopsy
- MRI and Mrs
- High-Resolution Respirometry
- Analyses of Lipid Peroxidation, Antioxidative Capacity and Oxidative Stress
- GC-MS
- Western Blotting
Fifteen male volunteers with type 2 diabetes were enrolled in this randomised, placebo-controlled, crossover clinical trial (ClinicalTrials.gov registration no. NCT04219215) but three volunteers were excluded prematurely. In total, 12 participants completed the study and data were analysed (ESM Table 1 and ESM Fig. 1). All participants were recruit...
The random allocation sequence (1:1) was generated by an experienced statistician at the German Diabetes Center (DDZ) (PB) using SAS software, version 9.3 (SAS Institute, Cary, NC, USA). Participants were randomly assigned to their treatment sequence by a person at the DDZ who was not involved in the conduct of the study. The randomisation list was...
The visits took place at an interval of 3 weeks. Each visit covered a period of 31 h, divided into four time periods (Fig. 1): basal (−24 h to −3 h); intervention (−3 h to +0 h); pre-clamp (+0 h to +2 h); and clamp (+2 h to +5 h). All participants arrived at the DDZ at 09:00 hours, after a 12 h overnight fast (−24 h to −3 h, basal period), for a 1 ...
Indirect calorimetry was performed in the canopy mode with Vmax Encore 29n (CareFusion, Höchberg, Germany) during the last 30 min of the basal, pre-clamp and clamp periods .
Skeletal muscle and WAT biopsy samples were taken from the vastus lateralis muscle and subcutaneous adipose tissue of the lower abdomen, respectively, as described before .
All MRI/MRS measurements were conducted on a 3.0-T MR scanner (Achieva X-series; Philips Healthcare, Best, the Netherlands). Intrahepatic lipid (IHL) and intramyocellular lipid (IMCL) contents were quantified by 1H-MRS. Hepatic γ-ATP and total inorganic phosphate (Pi) contents were measured with 31P-MRS using a 14 cm circular surface coil, as repor...
Ex vivo analysis of mitochondrial respiration was performed in permeabilised muscle fibres and WAT using high-resolution respirometry (Oxygraph-2k; Oroboros, Innsbruck, Austria), as previously described . Maximal mitochondrial respiration with electron input through CI and CII was determined after addition of malate (2 mmol/l), pyruvate (10 mmol/l)...
Concentrations of thiobarbituric acid reactive substances (TBARS) were assessed fluorometrically in serum and biopsy samples from subcutaneous fat and skeletal muscle according to the manufacturer’s instructions (BioTek, Bad Friedrichshall, Germany) . In skeletal muscle and WAT samples, total glutathione and oxidised glutathione (GSSG) contents wer...
Determination of atom per cent enrichment of blood [2H2]glucose was performed on a Hewlett Packard 6890 gas chromatograph equipped with a 25 m CPSil5CB capillary column (0.2 mm i. d., 0.12 μm film thickness; Chrompack/Varian, Middelburg, the Netherlands) and interfaced to a Hewlett Packard 5975 mass selective detector .
Expression levels of proteins of interest were assessed by western blot analysis, as described before . Data were normalised to housekeeping protein or total protein and expressed in arbitrary units. The following primary antibodies were purchased from Cell Signaling Technology and diluted 1:1000, unless differently specified: binding immunoglobuli...
Jun 28, 2021 · Insulin resistance is defined as the insufficient effect of insulin on tissues [24], and is measured using the HOMA-IR method [19]. Insulin sensitivity is determined by using the QUICKI score [19]. Insulin resistance is an important risk factor in the development of MetS and DM [25].
- Cemil Kahraman, Hüseyin Yaman
- 10.3906/sag-2011-76
- 2021
- Turk J Med Sci. 2021; 51(3): 1571-1577.
Sep 30, 2022 · Hyperbaric oxygen rapidly improves tissue-specific insulin sensitivity and mitochondrial capacity in humans with type 2 diabetes: a randomised placebo-controlled crossover trial September 2022 ...
Mar 1, 2015 · Hyperbaric oxygen rapidly improves tissue-specific insulin sensitivity and mitochondrial capacity in humans with type 2 diabetes: a randomised placebo-controlled crossover trial. Sarabhai T , Mastrototaro L , Kahl S , Bönhof GJ , Jonuscheit M , Bobrov P , Katsuyama H , Guthoff R , Wolkersdorfer M , Herder C , Meuth SG , Dreyer S , Roden M
- David Wilkinson, Mirjam Nolting, Mohd Kaisan Mahadi, Ian Chapman, Leonie Heilbronn
- 2015
Jan 31, 2020 · Studies have shown that hyperbaric oxygen enhances glucose and lipid metabolism in skeletal muscle, delaying the onset of type 2 diabetes and obesity . Exposure to mild hyperbaric oxygen increased skeletal muscle oxidative capacity . Nevertheless, it is unknown as to whether HBO improves insulin sensitivity and relative mechanism.
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and white adipose tissue (WAT) mitochondrial capacity. All participants and people assessing the outcomes were blinded. Results HBO decreased fasting blood glucose by 19% and increased whole-body, hepatic and WAT insulin sensitivity about one-third (p<0.05 vs CON).
