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Often, it is convenient to prepare a series of solutions of known concentrations by first preparing a single stock solution, as described in the previous section. Aliquots (carefully measured volumes) of the stock solution can then be diluted to any desired volume. In other cases, it may be inconvenient to weigh a small mass of sample ...
- Solution Stoichiometry
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- 2.5: Preparing Solutions
Solutions are often prepared by diluting a more concentrated...
- Chapter 12.1: Preparing Solutions
Dilution is also used to prepare solutions from substances...
- Solution Stoichiometry
Sep 12, 2021 · Solutions are often prepared by diluting a more concentrated stock solution. A known volume of the stock solution is transferred to a new container and brought to a new volume. Since the total amount of solute is the same before and after dilution, we know that. Co ×Vo = Cd ×Vd (2.5.1) (2.5.1) C o × V o = C d × V d.
Dilution is also used to prepare solutions from substances that are sold as concentrated aqueous solutions, such as strong acids. The procedure for preparing a solution of known concentration from a stock solution is shown in Figure 12.1.3. It requires calculating the number of moles of solute desired in the final volume of the more dilute ...
- Overview
- Performing a Basic Dilution
- Calculating Final Dilution Factor and Concentration
A dilution in chemistry is a process that reduces the concentration of a substance in a solution. A serial dilution is the repeated dilution of a solution to amplify the dilution factor quickly.
It’s commonly performed in experiments requiring highly
, such as those involving concentration curves on a
or when you are determining the density of bacteria. Serial dilutions are used extensively in experimental sciences like biochemistry, microbiology, pharmacology and physics.
Determine the proper dilution liquid.
The liquid that you will be diluting your substance in is very important. Many solutions will be diluted in distilled water, but this is not always the case. If you are diluting bacteria or other cells, you will likely want to dilute in culture media.
The liquid you choose will be used for every serial dilution.
If you’re unsure what liquid to use, ask for help or check online to see if other people have performed a similar dilution.
Prepare several test tubes with 9 mL of dilution liquid.
These tubes will serve as your dilution blanks.
The total dilution ratio can be determined by multiplying the dilution factor of each step leading up to the final step. This can be mathematically illustrated with the equation
is the total dilution factor and
For example, let’s say you did a 1:10 dilution of your liquid 4 times. Plug your dilution factor into the equation: D
= 10 x 10 x 10 x 10 = 10,000
The final dilution factor of the fourth tube in your serial dilution is 1:10,000. The concentration of your substance is now 10,000 times less than the original undiluted solution.
Determine the concentration of the solution following dilution.
- 729.1K
Jan 17, 2023 · Using a serial dilution, describe how you would prepare 10 mL of a 1%, 0.1% and 0.01% solution of NaOH. The stock solution of NaOH is 10%. Draw diagram as part of your description. Using the standard curve below, calculate the concentration of an unknown solution if its absorbance is 0.55. Figure 3.
So you need to mix 10 ml of 10X stock solution and 90 ml water to obtain 100 ml of working (diluted) solution. Example: When the concentration of stock solution concentration is given in a concentration unit e.g. 1 M stock solution. For example, you want to prepare 100 ml of 1 mM EDTA solution from 0.5M EDTA solution.
People also ask
How do you prepare a series of concentrations?
How to prepare a working solution of concentration and volume?
How can a solution of a known concentration be prepared?
How to prepare a solution of known concentration from a stock solution?
How do you calculate the concentration of a solution?
How is a solution prepared?
Add the solvent. Pour the stock solution into a graduated cylinder or other measuring container large enough to hold the final volume of the dilution. Slowly, mixing thoroughly as you go, add solvent until you reach the desired volume for the dilution (V2). Label and store the dilution.
