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Bacterial Growth Dynamics. Bacterial growth refers to an increase in bacterial numbers, not an increase in the size of the individual cells. In most bacteria, growth first involves increase in cell mass and number of ribosomes, then duplication of the bacterial chromosome, synthesis of new cell wall and plasma membrane, partitioning of the two ...
- 8.1: How Bacteria Grow
Figure 8.1.4 8.1. 4: The growth curve of a bacterial culture...
- Bacterial Growth and Reproduction
An individual bacterial cell will divide and eventually...
- 8.1: How Bacteria Grow
Jul 18, 2022 · Figure 8.1.4 8.1. 4: The growth curve of a bacterial culture is represented by the logarithm of the number of live cells plotted as a function of time. The graph can be divided into four phases according to the slope, each of which matches events in the cell. The four phases are lag, log, stationary, and death.
An individual bacterial cell will divide and eventually become a visible mass of cells known as a colony. If instead of a single cell, the solid media is initially populated with a large number of cells, confluent growth or a lawn of bacteria will be visible. Figure 2.2.5: Growth on solid medium.
The enlarged view shows the square within which bacteria (red cells) are counted. If the coverslip is 0.2 mm above the grid and the square has an area of 0.04 mm 2, then the volume is 0.008 mm 3, or 0.000008 mL. Since there are 10 cells inside the square, the density of bacteria is 10 cells/0.000008 mL, which equates to 1,250,000 cells/mL.
- Binary Fission
- Generation Time
- The Growth Curve
- Measurement of Bacterial Growth
- Plate Count
- Indirect Cell Counts
- Alternative Patterns of Cell Division
- Biofilms
The most common mechanism of cell replication in bacteria is a process called binary fission, which is depicted in Figure 1. Before dividing, the cell grows and increases its number of cellular components. Next, the replication of DNA starts at a location on the circular chromosome called the origin of replication, where the chromosome is attached ...
In eukaryotic organisms, the generation time is the time between the same points of the life cycle in two successive generations. For example, the typical generation time for the human population is 25 years. This definition is not practical for bacteria, which may reproduce rapidly or remain dormant for thousands of years. In prokaryotes (Bacteria...
Microorganisms grown in closed culture (also known as a batch culture), in which no nutrients are added and most waste is not removed, follow a reproducible growth pattern referred to as the growth curve. An example of a batch culture in nature is a pond in which a small number of cells grow in a closed environment. The culture density is defined a...
Estimating the number of bacterial cells in a sample, known as a bacterial count, is a common task performed by microbiologists. The number of bacteria in a clinical sample serves as an indication of the extent of an infection. Quality control of drinking water, food, medication, and even cosmetics relies on estimates of bacterial counts to detect ...
The viable plate count, or simply plate count, is a count of viable or live cells. It is based on the principle that viable cells replicate and give rise to visible colonies when incubated under suitable conditions for the specimen. The results are usually expressed as colony-forming units per milliliter (CFU/mL) rather than cells per milliliter be...
Besides direct methods of counting cells, other methods, based on an indirect detection of cell density, are commonly used to estimate and compare cell densities in a culture. The foremost approach is to measure the turbidity (cloudiness) of a sample of bacteria in a liquid suspension. The laboratory instrument used to measure turbidity is called a...
Binary fission is the most common pattern of cell division in prokaryotes, but it is not the only one. Other mechanisms usually involve asymmetrical division (as in budding) or production of spores in aerial filaments. In some cyanobacteria, many nucleoids may accumulate in an enlarged round cell or along a filament, leading to the generation of ma...
In nature, microorganisms grow mainly in biofilms, complex and dynamic ecosystems that form on a variety of environmental surfaces, from industrial conduits and water treatment pipelines to rocks in river beds. Biofilms are not restricted to solid surface substrates, however. Almost any surface in a liquid environment containing some minimal nutrie...
Oct 25, 2024 · Bacteria - Reproduction, Nutrition, Environment: Growth of bacterial cultures is defined as an increase in the number of bacteria in a population rather than in the size of individual cells. The growth of a bacterial population occurs in a geometric or exponential manner: with each division cycle (generation), one cell gives rise to 2 cells, then 4 cells, then 8 cells, then 16, then 32, and so ...
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Jun 4, 2019 · First, a growth law proposed that the bacterial cell volume depends exponentially on the growth rate and increases exponentially as growth proceeds 12. Second, when the generation time is shorter ...