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- As photosynthesis proceeds oxygen is released into the interior of the leaf, which changes the buoyancy--causing the disks to rise. Since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis.
cpb-us-e1.wpmucdn.com/blogs.cornell.edu/dist/3/1009/files/2015/09/Floating-Leaf-Disk-Brad-Williamson.pdfThe Floating Leaf Disk Assay for Investigating Photosynthesis
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Accumulation of O 2 on the disks causes them to float. The rate of production of O 2 can be affected by the intensity of the light source, but there is a maximum rate after which more light energy will not increase photosynthesis.
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- Condiment Diver
- Conducting The Floating Leaf Disk Assay
- Analyzing Your Results
- Changing Variables to Investigate Photosynthesis
Before you start testing different variables, conduct the leaf disk assay once to get familiar with the procedure. The video Measure Photosynthesis with Floating Leavesdemonstrates how to do each individual step. You will do all your experiments in triplicates after your test run; the following instructions are for the three trials of your experime...
For each cup, create a graph from your data table that shows the number of floating leaf disks over time. Plot the time on the x-axis and the number of floating leaf disks on the y-axis. An example...
Now that you are familiar with the leaf disk assay procedure and data analysis, you are ready to start your own investigations.Choose one variable that you want to investigate. You can find some suggestions in Table 2. The table also provides some possible variations for each variable that you can test.There are many factors that affect the rate of photosynthesis or how fast plants make oxygen. For example, you can change the temperature of the baking soda solution. Do your leaf disks start to float sooner in a colder or a warmer baking soda solution? Test how the intensity of the light affects photosynthesis.
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Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
Jul 11, 2023 · To measure the rate of photosynthesis, you will replace the air in the spongy mesophyll in your leaf disks with a liquid. This will cause the leaf disks to sink. Then you will put these leaf disks in water with dissolved CO 2 and measure the amount of time it takes for the leaf disks to float.
Jun 30, 2019 · Watch spinach leaf disks rise and fall in a baking soda solution in response to photosynthesis. The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose.
As photosynthesis proceeds oxygen is released into the interior of the leaf, which changes the buoyancy--causing the disks to rise. Since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis. !"#$%&"'()*.
