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      • Plant material will generally float in water. This is because leaves have air in the spaces between cells, which helps them collect CO 2 gas from their environment to use in photosynthesis. When you apply a gentle vacuum to the leaf disks in solution, this air is forced out and replaced with solution, causing the leaves to sink.
      www.exploratorium.edu/snacks/photosynthetic-floatation

      Photosynthetic Floatation | Exploratorium

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  4. www.sciencebuddies.org › science-fair-projectsUse Floating Leaf Disks to Study Photosynthesis

    In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. In the next step, a vacuum is used to replace the air pockets within the leaf structure with a baking soda (bicarbonate) solution. The baking soda provides the carbon dioxide that the leaf needs for photosynthesis.

  5. www.exploratorium.edu › snacks › photosyntheticPhotosynthetic Floatation | Exploratorium

    When you apply a gentle vacuum to the leaf disks in solution, this air is forced out and replaced with solution, causing the leaves to sink. When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis.

  6. www.sciencebuddies.org › stem-activitiesMeasure Photosynthesis with Floating Leaves | STEM Activity

    In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. In the next step, a vacuum is used to replace the air pockets within the leaf structure with a baking soda (bicarbonate) solution.

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  7. bio.libretexts.org › Learning_Objects › Laboratory2.1: Photosynthesis Protocol - Biology LibreTexts

    Jul 11, 2023 · Put your thumb over the tip of the syringe, and pull back slowly on the plunger to about the 10 mL mark. This creates a vacuum and pulls air out of the leaf.

  8. www.bradwilliamson.net › the-floating-disk-assayThe Floating Disk Assay to Study Photosynthesis–an update

    Sep 2, 2019 · Too much vacuum can damage the cells and cell spaces in the interior of the leaf and the procedure will likely not work. If you see the infiltration solution turning green you likely overdid the vacuum procedure.

    • What happens if you put a vacuum on a leaf disk?1
    • What happens if you put a vacuum on a leaf disk?2
    • What happens if you put a vacuum on a leaf disk?3
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    • What happens if you put a vacuum on a leaf disk?5

  9. cpb-us-e1.wpmucdn.com › blogs › distThe Floating Leaf Disk Assay for Investigating Photosynthesis

    While holding the vacuum, swirl the leaf disks to suspend them in the solution. Let off the vacuum. The bicarbonate solution will infiltrate the air spaces in the leaf causing the disks to sink. You will probably have to repeat this procedure 2-3 times in order to get the disks to sink.

  10. labsci.stanford.edu › images › Photosynthesis-T-Photosynthesis: How do plants get energy? - Stanford University

    The purpose of the vacuum is to force out all air in the inner spaces of the leaf disks. The solution will now have room to flood in and saturate the leaf disk.

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