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Measure the rate of photosynthesis in plants
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- Scientists have found an easy way to measure the rate of photosynthesis in plants. The procedure is called the floating leaf disk assay. In this plant biology project, you can use this procedure to investigate which factors affect the rate of photosynthesis.
www.sciencebuddies.org/science-fair-projects/project-ideas/PlantBio_p053/plant-biology/photosynthesis-leaf-disk-assay
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The procedure is called the floating leaf disk assay. In this plant biology project, you can use this procedure to investigate which factors affect the rate of photosynthesis. Do you think the light intensity, type of plant, or the temperature matter?
Sep 2, 2019 · For more than 25 years, I have promoted the floating leaf disk assay as one of the best ways for students to explore factors that affect photosynthesis. It’s replicable, it’s cheap, it’s accessible, and most of all it’s fun.
The Floating Leaf Disk Assay for Investigating Photosynthesis. Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable.
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In this lesson plan, students will use the floating leaf disk assay to explore photosynthesis and the factors that affect photosynthesis rates.
At any given point in this experiment, the number of floating leaf disks is an indirect measurement of the net rate of photosynthesis. In photosynthesis, plants use energy from the sun, water, and carbon dioxide (CO 2) from the air to store carbon and energy in the form of glucose molecules.
The leaf disk assay measures the rate of oxygen production in the leaf disks, which is a representation for the rate of photosynthesis. When you placed the leaf disks in your syringe, you probably noticed that they all floated on top of the solution.
The floating disk assay uses the rate at which oxygen is produced or consumed as a measure of the balance between the two reactions. Disks of leaf tissue are vacuum-infiltrated to replace intercellular air with liquid.