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- In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf. In the next step, a vacuum is used to replace the air pockets within the leaf structure with a baking soda (bicarbonate) solution. The baking soda provides the carbon dioxide that the leaf needs for photosynthesis.
www.sciencebuddies.org/science-fair-projects/project-ideas/PlantBio_p053/plant-biology/photosynthesis-leaf-disk-assay
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What is a floating leaf disk assay?
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What is a leaf disk assay?
The procedure is called the floating leaf disk assay. In this plant biology project, you can use this procedure to investigate which factors affect the rate of photosynthesis. Do you think the light intensity, type of plant, or the temperature matter?
Sep 2, 2019 · For more than 25 years, I have promoted the floating leaf disk assay as one of the best ways for students to explore factors that affect photosynthesis. It’s replicable, it’s cheap, it’s accessible, and most of all it’s fun.
In this fun plant biology activity, you will use a floating leaf disk experiment to measure how quickly plants can make oxygen from photosynthesis.
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At any given point in this experiment, the number of floating leaf disks is an indirect measurement of the net rate of photosynthesis. In photosynthesis, plants use energy from the sun, water, and carbon dioxide (CO 2 ) from the air to store carbon and energy in the form of glucose molecules.
The Floating Leaf Disk Assay for Investigating Photosynthesis. Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable.
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The floating leaf disk assay allows to indirectly determine the rate of photosynthesis. In this lesson plan, students will place 10 disks in the baking soda solution at the same time. A good way to collect data is to count the number of floating disks at the end of a fixed time interval; for example, after every minute until all disks are floating.
Jul 11, 2023 · In this section, students learn how to use the floating leaf disk method to measure the rate of net photosynthesis (i.e. the rate of photosynthesis minus the rate of cellular respiration). They compare the rate of net photosynthesis in water vs. a solution of sodium bicarbonate.