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Measure the rate of oxygen production
- Without the use of extensive laboratory equipment, the rate of photosynthesis can be determined indirectly by conducting a floating leaf disk assay to measure the rate of oxygen production (Figure 2). In the floating leaf disk assay, 10 or more leaf disk samples are punched out of a leaf.
www.sciencebuddies.org/science-fair-projects/project-ideas/PlantBio_p053/plant-biology/photosynthesis-leaf-disk-assay
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Can a floating leaf disk test be used for photosynthesis?
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What is a leaf disk assay?
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The procedure is called the floating leaf disk assay. In this plant biology project, you can use this procedure to investigate which factors affect the rate of photosynthesis. Do you think the light intensity, type of plant, or the temperature matter?
The leaf disk assay measures the rate of oxygen production in the leaf disks, which is a representation for the rate of photosynthesis. When you placed the leaf disks in your syringe, you probably noticed that they all floated on top of the solution.
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Sep 2, 2019 · For more than 25 years, I have promoted the floating leaf disk assay as one of the best ways for students to explore factors that affect photosynthesis. It’s replicable, it’s cheap, it’s accessible, and most of all it’s fun.
At any given point in this experiment, the number of floating leaf disks is an indirect measurement of the net rate of photosynthesis. In photosynthesis, plants use energy from the sun, water, and carbon dioxide (CO 2) from the air to store carbon and energy in the form of glucose molecules.
The Floating Leaf Disk Assay for Investigating Photosynthesis. Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable.
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In this lesson plan, students will use the floating leaf disk assay to explore photosynthesis and the factors that affect photosynthesis rates.
The Floating Leaf Disk Assay for Investigating Photosynthesis. Brad Williamson. Introduction: Trying to find a good, quantitative procedure that students can use for exploring photosynthesis is a challenge. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable.
