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In a process called photosynthesis, plants convert light energy, water, and carbon dioxide into oxygen and sugar. They can then use the sugar as an energy source to fuel their growth. Scientists have found an easy way to measure the rate of photosynthesis in plants. The procedure is called the floating leaf disk assay.
When you see tiny bubbles forming on the leaf disks during this experiment, you’re actually observing the net production of O 2 gas as a byproduct of photosynthesis. Accumulation of O 2 on the disks causes them to float.
During photosynthesis, plants convert light, water, and carbon dioxide from the air into oxygen and sugars. In this activity, you will be able to observe the oxygen production in leaves by doing a floating leaf disk experiment. You can also find out how quickly plants produce oxygen, and what variables affect photosynthesis.
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Jul 11, 2023 · You will use the "floating leaf disk" method to measure the rate of photosynthesis. To begin, cut several disks from a spinach leaf and put these leaf disks in a cup of water. 1. Do your leaf disks float? Use the information in this diagram of a cross-section of a leaf to explain why a leaf disk would float. 2. Where does photosynthesis occur ...
Jun 30, 2019 · The leaf disks intake carbon dioxide from a baking soda solution and sink to the bottom of a cup of water. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. Oxygen released from the leaves forms tiny bubbles that cause the leaves to float.
- Anne Marie Helmenstine, Ph.D.
Jul 26, 2022 · As oxygen is produced by photosynthesis, it comes out of solution and infiltrates the leaf tissue, replacing some of the water. This decreases the density of the disks, and they begin to float. The number of disks that float per unit of time gives a measure of the rate of photosynthesis.
Sep 2, 2019 · Leaf disks float, normally. When the air spaces are infiltrated with the solution the overall density of the leaf disk increases and the disk sinks. The infiltration solution includes a small amount of Sodium bicarbonate. Bicarbonate ion serves as the carbon source for photosynthesis.
