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The implementation of systems controlling environmental conditions, such as perfusion pumps for continuous supply of nutrients and diurnally adjusted external light sources, allowed long-term live and developmental imaging of plants within experiments lasting for hours or even days (reviewed in Ovečka et al., 2018). Of particular interest to plant scientists is a geometry of LSFM systems ...
Sep 29, 2021 · In particular, propidium iodide (PI) is a red fluorescent dye that labels pectins in the cell wall and is often used in live imaging approaches to label cell contour (Kierzkowski et al., 2019; Sede et al., 2020); however, it is toxic to cells and affect growth, thus limiting long-term live cell imaging. Alternatively, membrane dyes or fluorescently tagged plasma membrane proteins are often ...
Jan 28, 2021 · Here, we establish long-term live-cell imaging of single Arabidopsis cells undergoing proto-xylem trans-differentiation, resulting in spiral wall patterns, to understand microtubule re ...
- René Schneider, René Schneider, Kris van ’t Klooster, Kelsey L. Picard, Kelsey L. Picard, Jasper van...
- 2021
Sep 11, 2021 · Such remarkable advances include the functional imaging of tissues and organs by lightsheet and two-photon microscopy for long-term three-dimensional and deep-tissue imaging. Super-resolution and single-particle imaging, including artificial intelligence–based denoizing, are other major trends that have been reviewed elsewhere ( Schubert 2017 , Cui et al. 2018 , Komis et al. 2018 , Krull et ...
- Tetsuya Higashiyama, Tetsuya Higashiyama, Alexis Maizel, Rüdiger Simon
- 2021
Mar 9, 2023 · It has been suggested that, for most plant imaging applications, there is little need for the absolute quantification of individual metabolites, since relative amounts usually provide sufficient information (Dueñas et al., 2019). With or without derivatization, plant metabolomes harbor a wealth of structural isomers and/or isobaric species.
- 2
- 238, Issue5
- 09 March 2023
Jul 23, 2015 · To study developmental growth in plants, long-term imaging experiments have typically used confocal laser-scanning microscopy (CLSM), spinning-disc (SD) and epifluorescence platforms. Excitation ...
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Mar 1, 2023 · Long-term imaging of plants requires a suitable light source to support plant growth. The ability to illuminate plants with specific light wavelengths is essential to study light signaling pathways. Both fluorescent and white light-emitting diodes (LEDs) can be used to grow plants, and an array of filters can modify the light source's spectral qualities.
